Biology Lab

Lab 2 Cell Structure and Function BIO101L

 

 

Student Name: Click here to enter text.

Access Code (located on the lid of your lab kit): Click here to enter text.

Pre-Lab Questions

1. Identify three major similarities and differences between prokaryotic and eukaryotic cells.

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2. Where is the DNA housed in a prokaryotic cell? Where is it housed in a eukaryotic cell?

 

 

3. Identify three structures which provide support and protection in a eukaryotic cell.

 

Experiment 1: Identifying Cell Structures

Post-Lab Questions

1. Label each of the arrows in the following slide image:

Structure Identity
A Click here to enter text.
B Click here to enter text.
C Click here to enter text.
D Click here to enter text.

 

 

2. What is the difference between the rough and smooth endoplasmic reticulum?

 

 

3. Would an animal cell be able to survive without mitochondria? Why or why not?

 

 

4. What could you determine about a specimen if you observed a slide image showing the specimen with a cell wall, but no nucleus or mitochondria?

 

 

5. Hypothesize why parts of a plant, such as the leaves, are green, but other parts, such as the roots, are not. Use scientific reasoning to support your hypothesis.

 

Experiment 2: Create a Cell

Post-Lab Questions

1. What cell structures did you place in the plant cell that you did not place in the animal cell?

 

 

2. Is there any difference in the structure of the two cells?

 

 

3. What structures do cells have for support in organisms that lack cell walls?

 

 

4. How are organelles in a cell like organs in a human body?

 

 

5. How does the structure of a cell suggest its function? List three examples.

 

6. In the table below, list the items you used to represent the various organelles in your ANIMAL cell. Provide a brief rationale explaining why you selected each item.

Item Organelle Rationale
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7. Insert picture of your ANIMAL cell with your name and access code handwritten in the background.

 

8. In the table below, list the items you used to represent the various organelles in your PLANT cell. Provide a brief rationale explaining why you selected each item.

Item Organelle Rationale
Click here to enter text. Click here to enter text. Click here to enter text.
Click here to enter text. Click here to enter text. Click here to enter text.
Click here to enter text. Click here to enter text. Click here to enter text.
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9. Insert picture of your PLANT cell with your name and access code handwritten in the background.

 

 

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Blood Typing

To Access the Virtual Lab: https://www.classzone.com/books/hs/ca/sc/bio_07/virtual_labs/virtualLabs.html

Click on the “Blood Typing” 

Introduction

1. Summarize the problem you are trying to solve.

2. What is the purpose of this investigation?

Explore Lab

3. What types of serum are available?

What to the antibodies in the serums bind to?

4. What are the disposable micropipettes used for?

Procedure

5. Prediction Chart (indicate YES or NO if you think the blood will clump when exposed to the antibodies)

 

Blood Type

Anti-A Antibody

Anti-B Antibody

Anti-Rh Antibody

 

A

 

B

 

AB

 

O

 

Rh+

 

Rh-

6. Observation Chart

 

Blood Sample

Clumps in Response to

Anti-A

Clumps in Response to

Anti-B

Clumps in Response to

Anti-Rh

Type

 

1

 

2

 

3

 

4

Analyze and Conclude

7. Which, if any, of the blood samples tested cna the patient with type B+ blood receive? Explain why.

8. Explain how you were able to use your knowledge of how different types of blood react with Anti-A, Anti-B, and Anti Rh antibodies to determine the blood types of the four samples.

9. If a person has Type A blood, he or she would have antibodies for what blood type?

10. Why is type O Negative blood known as the universal donor? Why is O Positive not a universal donor?

11. If a person has Type O blood, what type(s) of blood would she not be able to receive?

12. Is knowing the ABO blood type of a potential blood donor enough to determine a suitable match? Explain your answer.

13. What might happen if someone with Type A received a transfusion of Type B blood?

 
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Anthropology

Beginning Thoughts on Anthropology, Culture & Cultural Diversity

1.)What ideas or images that come to mind if someone says “Anthropology” or “anthropologist”? What has shaped your ideas about what Anthropology is or what Anthropologists do?

2.)What IS Anthropology? And what are the four sub-fields of Anthropology?

3.)Some contemporary archaeologists focus on studying modern human waste….including e-waste. If someone were to study YOUR waste (trash) …and e-waste what would they learn about you? Your diet? Your lifestyle?

4.) What are some cultural adaptations human beings have to better allow them to survive in their environment? Are there negative effects of these adaptations? If so, do the benefits outweigh those negative effects?

5.)American anthropologist Ralph Linton once said “The last thing a fish would ever notice would be water.” (Ralph Linton, 1936) How is this relevant and applicable to a discussion on “culture”?

6.) Polish anthropologist, Bronislaw Malinowski who is credited with inventing the anthropological method of intensive fieldwork, wrote in his journal about his fieldwork in the Trobriand Islands.In his diary he wrote” Imagine yourself suddenly set down surrounded by all your gear, alone on a tropical beach close to a native village, while the launch or dinghy which has brought you sails away out of sight.” What is Malinowski describing? Have you ever had an analogous experience in Philadelphia (or elsewhere)?

7.)Clifford Geertz, one of the most influential American anthropologists in the last 40 years, said “The locus of study is not the object of study. Anthropologists don’t study villages (tribes, neighborhoods…) they study in villages.” What do you think he meant? (And what happens if you substitute college for village?)

8.) If I were to ask you to provide a “socio-cultural analysis” of this classroom…where would you begin? Can you identify 10 ways that you might consider “diversity” within this classroom? (on campus, in the city, in the U.S. or in the world?)

9.) How do you think about diversity? i.e.as a problem? A challenge? An asset? Explain

10.) What do you think is bigger…a nation-state or a culture? Briefly explain.

 
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BIO 100 LAB 7

Compose answers to the questions below and save the file as a backup copy in the event that a technical problem is encountered while attempting to submit the assignment. Make sure to run a spell check.

You will be submitting your answers to the lab assignment in two parts. The first part of the lab assignment consists of the laboratory exercise questions. The second part of the lab assignment is the application question. The first textbox on the submission page corresponds to the first part of the lab. Be sure to paste the laboratory exercise questions, with your answers, into this textbox. The second textbox on the submission page will be for your response to the application question.

 

LABORATORY EXERCISE QUESTIONS

 

~~1. What is an allele? (1 point)

~~2. Give an example of a genetic trait and two allele phenotypes for the gene that determines the trait. (3 points)

a. Genetic Trait

b. Allele phenotype #1

c. Allele phenotype #2

~~3. Compare and contrast the terms phenotype and genotype. (4 points)

~~4. List the allele combinations (gamete possibilities) that can be formed by an individual with the following genotype: AABB (1 point)

~~5. List the different allele combinations (gamete possibilities) that can be formed by an individual with the following genotype: AaBb. (4 points)

a.

b.

c.

d.

~~6. Given: P = purple flowers and p = white flowers and P is dominant over p.

a. What is the phenotypic ratio of offspring from a cross between Pp x pp? (2 points)

b. In a population with 160 individuals how many will be homozygous? (2 points)

c. In that same population, how many will be purple? (2 points)

 

~~7.

a. Which of Mendel’s laws is illustrated in a dihybrid cross? (1 point)

b. What does this law state? (1 point)

~~8. A horticulturist has a purple plant and a white plant. The horticulturist knows that purple is dominant over white. When they are bred, all of the resulting offspring are purple. What is the most likely genotype of the parent or original purple plant? (2 points)

~~9. A horticulturist runs a test cross with an offspring (F1 generation) purple plant from Question 8. The phenotypic frequencies of the resulting offspring are 50% white and 50% purple. What is the true genotype of this offspring (F1 generation) purple plant? (2 points)

~~10. What is the probability of a cross resulting in white offspring when two heterozygous purple pea plants (e.g. Pp x Pp) are bred? What is the genotype for this offspring? (4 points)

~~11. Compare the ratios calculated in Exercises 5 and 6 (coin toss activity) to Mendel’s ratios. Were they close? Pose a possible explanation for why the ratios may not be exactly the same. (4 points)

~~12. Refer to the data on the corn kernel color ratio from Part II of the lab.

a. What was the phenotypic ratio from Step 1? (2 points)

b. What was the phenotypic ratio from Step 2? (2 points)

c. What was the phenotypic ratio from Step 3? (Remember there were four possible types for this part of the lab.) (2 points)

d. Explain why your numbers did not come out exactly. (2 points)

 

 

 

 

 

 

 

 

 

 

~~13. Recall from the background information that purple kernels are dominant and yellow kernels are recessive. The second ear of corn was the result of crossing two heterozygous ears of corn male purple (Pp x Pp). This is represented by the Punnett square below. Complete the Punnett square by writing the correct letters that correspond to each number indicated in the table. (4 points)

  P p
P 1 2
p 3 4

1.

2.

3.

4.

~~14. Once the Punnett square for Question 13 is complete, calculate the ratio of purple and yellow kernels (recall that if the dominant trait is present, it will be expressed).

h. What is the ratio of purple to yellow kernels based on the Punnett square? (2 points)

h. How did this compare to the ratio obtained from counting the corn kernels for ear number two in part II of the lab? (2 points)

 

 

 

 

 

 

 

 

 

 

~~15. Recall from the background information that purple kernels are dominant and yellow kernels are recessive. Also recall that smooth kernels are dominant and wrinkled kernels are recessive. The third corn ear was the result of crossing a male ear of corn with the following gametes: PpSs, with a female ear of corn with the same gametes: PpSs. This is represented by the Punnett square, below. Complete the Punnett square by writing the correct letters that correspond to each number indicated in the table (for example, PPSS or ppss). (8 points)

  PS Ps pS ps
PS 1 2 3 4
Ps 5 6 7 8
pS 9 10 11 12
ps 13 14 15 16

1.

2.

3.

4.

5.

6.

7.

8.

9.

10.

11.

12.

13.

14.

15.

16.

~~16. Once the Punnett square for Question 15 is complete, calculate the ratio of corn kernel varieties (recall that if the dominant trait is present, it will be expressed).

a. What is the ratio of kernels based on the Punnett square? (2 points)

b. How does this compare to the ratio obtained from counting the corn kernels? (2 points)

~~17. What are the genotypic and phenotypic ratios for kernel color and kernel texture for a dihybrid cross between PpSS x Ppss? (4 points)

c. genotypic ratio

d. phenotypic ratio

~~18. Using the results from the Punnett Square in Question 15, answer the following question: In a population of 240 corn kernels, how many will have smooth kernel texture? (2 points)

~~19. In a flower garden, a horticulturist is growing purple and white pansies. The horticulturist notices that a new pansy has sprouted. When it finally flowers, the pansy is lavender. Explain how this happened. (4 points)

~~20. With a botanist’s help, an individual decides to cross the lavender pansy with the white pansy. Will this result in any purple pansies? Explain. (4 points)

~~21. State an industrial concern/importance for knowing the genetic makeup of an organism. Explain why it is important in your example. (5 points)

 

APPLICATION QUESTION

 

~~22. (Application) How might the information gained from this lab pertaining to genetics be useful to you, or how can you apply this knowledge to your everyday life as a non-scientist? The application will be graded according to the rubric below. (20 points)

 
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