Biology blood typing lab report

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BIOL 102: Lab 9

Simulated ABO and Rh Blood Typing

Objectives:

After completing this laboratory assignment, students will be able to:

• explain the biology of blood typing systems ABO and Rh

• explain the genetics of blood types

• determine the blood types of several patients

Introduction:

Before Karl Landsteiner discovered the ABO human blood groups in 1901, it was thought that all blood was the

same. This misunderstanding led to fatal blood transfusions. Later, in 1940, Landsteiner was part of a team

who discovered another blood group, the Rh blood group system. There are many blood group systems known

today, but the ABO and the Rh blood groups are the most important ones used for blood transfusions. The

designation Rh is derived from the Rhesus monkey in which the existence of the Rh blood group was

discovered.

Although all blood is made of the same basic elements, not all blood is alike. In fact, there are eight different

common blood types, which are determined by the presence or absence of certain antigens – substances that

can trigger an immune response if they are foreign to the body – on the surface of the red blood cells (RBCs

also known as erythrocytes).

ABO System:

The antigens on RBCs are agglutinating antigens or agglutinogens. They have been designated as A and B.

Antibodies against antigens A and B begin to build up in the blood plasma shortly after birth. A person

normally produces antibodies (agglutinins) against those antigens that are not present on his/her erythrocytes

but does not produce antibodies against those antigens that are present on his/her erythrocytes.

• A person who is blood type A will have A antigens on the surface of her/his RBCs and will have

antibodies against B antigens (anti-B antibodies). See picture below.

• A person with blood type B will have B antigens on the surface of her/his RBCs and will have antibodies

against antigen A (anti-A antibodies).

• A person with blood type O will have neither A nor B antigens on the surface of her/his RBCs and has

BOTH anti-A and anti-B antibodies.

• A person with blood type AB will have both A and B antigens on the surface of her/his RBCs and has

neither anti-A nor anti-B antibodies.

The individual’s blood type is based on the antigens (not the antibodies) he/she has. The four blood groups

are known as types A, B, AB, and O. Blood type O, characterized by an absence of A and B agglutinogens, is

the most common in the United States (45% of the population). Type A is the next in frequency, found in 39%

of the population. The incidences of types B and AB are 12% and 4%, respectively.

 

 

 

 

 

 

 

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Table 1: The ABO System

Blood Type

Antigens on RBCs

Antibodies in the Blood

Can GIVE Blood to Groups:

Can RECEIVE Blood from Groups:

A A Anti-B A, AB O, A

B B Anti-A B, AB O, B

AB A and B Neither anti-A

nor anti-B AB O, A, B, AB

O Neither A nor

B Both anti-A and anti-B

O, A, B, AB O

 

Blood Typing: Process of Agglutination

Blood typing is performed with antisera containing high levels of anti-A and anti-B antibodies/agglutinins. The

simple test is performed as follows:

 

Several drops of each kind of antiserum are added to separate samples of

blood. If agglutination (clumping of erythrocytes) occurs only in the

suspension to which only anti-A serum was added, the blood type is A. If

agglutination occurs only in the anti-B mixture, the blood type is B (see image).

Agglutination in both samples indicates that the blood type is AB. The absence

of agglutination indicates that the blood type is O.

 

 

 

 

Table 2: Agglutination Reaction of ABO Blood-Typing Sera

Reaction to Anti-A Serum Reaction to Anti-B Serum Blood Type

Agglutination (clumping)

No agglutination (no clumping)

Type A

No agglutination (no clumping)

Agglutination (clumping)

Type B

Agglutination (clumping)

Agglutination (clumping)

Type AB

No agglutination (clumping)

No agglutination (clumping)

Type O

 

 

 

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Rh System

In the period between 1900 and 1940, a great deal of research was done to discover the presence of other

antigens on human red blood cells. In 1940, an antigen designated as Rh factor, was discovered. Although it

exists as six antigens, the D factor is responsible for the Rh+ condition. The Rh factor is found in 85% of

Caucasians, 94% of African-Americans, and 99% of Asians. An individual who possesses these antigens is

designated as Rh+; an individual who lacks them is designated Rh-. The anti-Rh antibodies of the systems are

not normally present in the plasma, but anti-Rh antibodies can be produced upon exposure and sensitization to

Rh antigens.

The genetics of the Rh blood group system is complicated by the fact that more than one antigen can be

identified as the result of the presence of a given Rh gene. Initially, the Rh phenotype was thought to be

determined by a single pair of alleles. However, there are at least eight alleles for the Rh factor. For the

purpose of simplicity, consider one allele: Rh+ is dominant over Rh-. Thus a person with Rh+/Rh-

heterozygous genotype has Rh+ blood.

Importance of Blood Typing

Early attempts to transfer blood from one person to another produced varied results. If incompatible blood

types are mixed, erythrocyte destruction, agglutination and other problems can occur. For instance, if a person

with Type B blood is transfused with blood type A, the recipient’s anti-A antibodies will attack the incompatible

Type A erythrocytes. The Type A erythrocytes will be agglutinated, and hemoglobin will be released into the

plasma. In addition, incoming anti-B antibodies of the Type A blood may also attack the Type B erythrocytes of

the recipient with similar results. This problem may not be serious, unless a large amount of blood is

transfused.

The ABO blood groups and other inherited antigenic characteristics of red blood cells are often used in

medico-legal situations involving identification or disputed paternity. In paternity cases a comparison of the

blood groups of mother, child, and alleged father may exclude the man as a possible parent of the child. For

example, a child of blood type AB whose mother is Type A could not have as a father a man whose blood

group is Type O. Blood typing does not prove that an individual is the father of a child, it merely indicates

whether or not he is a possible parent.

 

 

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The Genetics of Blood Types

Alleles are different versions of the same gene that can occupy the same locus (gene location on a

chromosome). There are usually two alleles of each gene. Humans have two copies of each gene because

they receive one copy from their mother and one copy from their father. If they receive two of the same alleles,

they are considered homozygous. If they have two different alleles, they are considered heterozygous. Alleles

can also be dominant and recessive. Alleles are dominant when the presence of one allele is sufficient to

express the trait and recessive when two copies of the allele must be present to express the trait.

The human blood types A, B, AB, and O are inherited by multiple alleles. Multiple alleles refer to three or more

genes that occupy a single locus. In the case of blood types, there are three versions of the gene which

encodes agglutinogens: A, B and O. The A and B alleles are both dominant and are considered co-dominant.

The O allele is recessive to both A and B alleles.

The alleles for blood types are often designated with the letter I with a subscript:

• The A allele is designated IA and codes for the synthesis of agglutinogen A

• The B allele is designated IB and codes for synthesis of agglutinogen B

• The O allele is designated i or IO and does not produce any antigens.

The phenotypes listed in the table below are produced by the combinations of the three different alleles IA, IB,

and IO.

 

 

 

 

 

 

 

 

Using Punnett Squares to Determine Future Genetic Combinations

A Punnett square is a chart which shows/predicts all possible gene combinations in a cross of parents (whose

genes are known). Punnett squares are named for an English geneticist, Reginald Punnett. He discovered

some basic principles of genetics, including sex linkage and sex determination. He worked with the feather

color traits of chickens in order to quickly separate male and female chickens.

Punnett squares can also be used to predict the blood type of future offspring between two people with a

known genotype. When creating the chart, the first step is to designate letters for dominant and recessive

alleles. It has been previously mentioned that A (IA) and B (IB) are both dominant alleles while O (i) is

recessive; therefore, this step is complete. The second step is to write the genotype (genetic combination) of

each parent and the third step is to list the alleles that each parent can contribute. If the parent is homozygous

(both alleles are either dominant or recessive), then she/he can only pass on the dominant allele that she/he

possesses. If the parent is heterozygous (one allele is dominant and the other allele is recessive or she/he has

both A and B dominant alleles), then he/she can pass on either allele. The fourth step is to draw the Punnett

square (one large square containing four smaller squares) and write the possible genes of one parent along

Table 3: Phenotypes and Possible Genotypes

Phenotype Possible Genotypes

A IA IA (homozygous dominant A) OR

IA i (heterozygous A)

B IB IB (homozygous dominant B) OR

IB i (heterozygous B)

AB IA IB (co-dominant AB)

O ii (homozygous recessive O)

 

 

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the top and the possible genes of the other parent along the left side. The fifth step is to fill the smaller square

by transferring in the parental letter above the square and the parental letter to the left of the square. The sixth

step is to list all of the possible genotypes (the combinations in each small square) and resultant phenotypes

(physical trait). Figure 1 below is of a cross (mating) between a person who is homozygous dominant A (type

A) and a person who is homozygous recessive (type O).

 

 

 

 

 

 

All of the children would have a heterozygous A genotype and blood type A phenotype.

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

IA IA

i IA i IA i

i IA i IA i

 

 

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LAB DATASHEET Purpose Each group will perform blood typing analyses to determine the unknown blood types of four patients using the

ABO and Rh factor systems.

 

Procedure

1. Obtain four (4) blood typing trays and use the wax pencil to label them as follows: P1, P2, P3, and P4.

2. Place five (5) drops of Patient 1 Simulated Blood Sample in each well (A, B, and Rh) of the P1 tray.

a. Place three (3) drops of Anti-A Simulated Serum in Well A and mix the blood and serum with a stirring

stick for ten (10) seconds.

b. Place three (3) drops of Anti-B Simulated Serum in Well B and mix the blood and serum with a stirring

stick for ten (10) seconds.

c. Place three (3) drops of Anti-Rh Simulated Serum in Well Rh and mix the blood and serum with a

stirring stick for ten (10) seconds.

d. Carefully examine each well to determine if the simulated blood in each well has clumped

(agglutinated). Record your results and observations in Table 4.

3. Place five (5) drops of Patient 2 Simulated Blood Sample in each well (A, B, and Rh) of the P2 tray.

Repeat directions ā€œa-dā€ listed under Step 2.

4. Place five (5) drops of Patient 3 Simulated Blood Sample in each well (A, B, and Rh) of the P3 tray.

Repeat directions ā€œa-dā€ listed under Step 2.

5. Place five (5) drops of Patient 4 Simulated Blood Sample in each well (A, B, and Rh) of the P4 tray.

Repeat directions ā€œa-dā€ listed under Step 2.

6. Thoroughly rinse all trays and stirring sticks and return to their proper location.

 

 

 

Table 4: Agglutination Reaction Results

 

Anti-A

Serum

(+ or -)

Anti-B Serum

(+ or -)

Anti-Rh

Serum

(+ or -)

Observations

(Clumping?) Blood Type

Patient 1:

Mr. Smith

Patient 2:

Mr. Jones

Patient 3:

Mr. Green

Patient 4:

Ms. Brown

 

 

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Analysis of Results

1. What ABO agglutinogens are present on the red blood cells of Mr. Green’s blood?

2. What ABO agglutinins are present in the serum of Mr. Green’s blood?

3. If Mr. Jones needed a transfusion, what ABO type(s) of blood could he safely receive?

4. If Ms. Brown were serving as a donor, what ABO blood type(s) could receive her blood safely?

5. Why is it necessary to match the donor’s and the recipient’s blood before a transfusion is given?

 
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Statistical Biology Lab Report

UTSC Journal of Plant Biology

BIO A01 2018- Fall; 1(1): 1-6

Insert Principal Author’s Name/Student Number

Paper title (The title should be specific and concise-Do not use ā€œFormal Lab Reportā€ in the title. All words except the first word should be in lower case-except for proper nouns.)[endnoteRef:1] [1: Template modified from the following resources: ā€œManuscript Template,ā€ Science Publishing Group, The Open Access Publisher 2012 URL http://www.sciencepublishinggroup.com/journal/guideforauthors.aspx?journalid=173; Guidelines for Writing Scientific Papers, Honors Organismal Biology Laboratory (no date), URL http://www.bms.bc.ca/resources/library/pdf/GuidelinesScientificPapers.pdf; Guidelines for Writing a Scientific Paper, Maloy 2001, URL http://www.sci.sdsu.edu/~smaloy/MicrobialGenetics/topics/scientific-writing.pdf; and Writing a Scientific Research Paper, Massachusetts Institute of Technology 2000. URL http://umech.mit.edu/freeman/6.021J/2000/writing.pdf. ]

Author’s Name (Principal Author), 1, Author’s Name (Bench-mate 1), 1 Authors Name (Bench-mate 2), 1 Author’s Name (Bench-mate 3), 1 Author’s Name (Bench-mate 4), 1 Author’s Name1 (Bench-mate 5) 1 – If you do not know your bench-mates names, please write your name + 4 other BIOA01 students in PRAXX

1Dept. of Biological Sciences, University of Toronto Scarborough, Toronto, Canada

UTSC BIOA01 Lab PRAXX, BENCHX:

PRAXX TA:

Abstract: An abstract is a one-paragraph summary of your report. It should begin with a few introductory remarks that introduce the significance of the study. It should include (in this order) the background of the study (1-3 sentences), mentioning of the study system/species/object (1 sentence), the question investigated (1 sentence), the general methods used (1 sentence), the principle results (1 sentence) and the conclusions (1 sentence). The reader should be able to determine the major points of your report without having to read further. The language should be concise and no citations should be included in the abstract. The abstract is located at the beginning of your report, however it is usually written once you have finished writing your paper.

Keywords: Include at least 3 keywords or phrases (specific to your paper), which must be separated by commas to differentiate them.

Introduction [Page limit-1 page]

This template is set up to provide you with an example of the format expected for your Formal Lab Report (FLR). The template provides you with the specifications needed for preparing your FLR. You can save this file as a separate document and type your report directly into the template. You can then submit your edited version of this file to Quercus. Please note that Quercus will only accept Word (.docx or .doc) files or PDF (.pdf) files.

The introduction provides a context for the research. This section should include the following: 1) Description of the current state of knowledge or understanding at the beginning of your investigation (i.e., background information synthesized from the existing literature – think about what information readers would need to know to be able to understand your lab report); 2) Background information about study species used; 3) The purpose of the experiment and/or the question being asked; 4) Hypothesis/hypotheses written as statements. Null hypotheses may be included here; 5) Brief description of the approach being used to test your hypothesis/hypotheses statement; 6) Predictions written as explanatory statements (ā€œIf…thenā€) that focus only on experimental treatment groups (not controls) and are backed up with relevant references.

It is imperative that you include properly formatted in-text citations to support all non-original ideas within your introduction. Failure to include in-text citations will result in a grade penalty and could possibly lead to an academic offence.

Materials and Methods [Page limit – 1/2 page- 1 page]

The purpose of this section is to describe the experimental procedures, including any controls. This section should be written in the past tense (and first-person if applicable); the remainder of the paper should be written in the present tense. The description should be complete enough to allow someone to repeat your work. The Methods section should describe the chronological process that you used to complete the research, how all of the data was collected, and a short description of the statistical analyses you completed. It should be written in complete sentences, not bulleted lists. Do not include lab coat, gloves, or safety goggles in your materials description-the use of personal safety equipment is assumed.

Be certain to include any software used to produce graphs and analyze data (e.g., Excel, GraphPad). Also, be certain to include an in-text citation of the lab manual in this section (and a corresponding complete reference in your reference section) but summarize the methods in your own words.

Results [Page limit – 1 ½ – 2 pages (written ½-1 page, figure ½ page, table ½ page)]

The results section describes the results of, but DOES NOT interpret, your experiment. You should present your table and figure in this section. The ā€˜Results’ section should always begin with text and not your table and figure. You should describe your findings to the reader – you should refer the reader to your table and figure in your results description (e.g., see Table 1 or Figure 1). By referring to your table and figure appropriately, you can concisely present your results in several paragraphs. If you do not refer to the appropriate figure or table in your results section, you will be penalized.

For the purpose of this report, your table and figure should be embedded within your results section. Be certain that there is not a page break in the middle of your table or figure and do not wrap text around the outside of the table and figure. (Note that some journals require that the tables and figures be included following the reference section.) The table caption should appear above the table, whereas the figure caption should appear below the figure. Insert your table and figure after they are cited in the text.

Be sure to record all your class data on the Table 3.2 in your lab manual. You will need these data to do the statistical analysis to produce the Table and Figure for your ā€˜Results’ section of your Formal Lab Report. See tips for the Table caption below.

Table 1: Your caption should be above your table and include details of what is included in your table. The information in your caption/table should be complete enough and presented in a way that the reader can easily understand the information presented without referring to the text of your report.

INSERT TABLE HERE – Your Statistical Worksheets should not be used for your Table in your Formal Lab Report. You must select information from your Worksheets to make a Table for your FLR. Your Table should include the following columns for each t-test comparison. You will be comparing each of the four treatment groups (light intensity in lumens) with the negative control (dark), as well as the positive control (outside light). Thus, you will have 8 comparisons.

Your Table should include the following columns for each t-test comparison:

a. n

b. critical t- value

c. calculated t-value

d. df

e. actual p-value (p > 0.05 or p < 0.05 or p = 0.05)

f. conclusion (did you reject or fail to reject the null hypothesis?)

INSERT FIGURE HERE – Prepare a bar graph with standard deviation error bars using the total oxygen produced (ml) for your complete data set (posted on Quercus for your lab practical). This means that the columns will be an average of all 8 values for each control and each experimental treatment group. You will have a total of 6 bars in your bar graph. Treatments should be shown as categories on the x- axis, mean total oxygen produced (ml) should be on the y- axis. The controls and the 4 treatments should be discernable by clear labels on the x-axis.

Note: If treatments cannot be discerned from your figure, you will be penalized.

Figure 1: Your caption should be below your figure and include details of what is depicted in your graph. The information in your caption/graph should be complete enough and presented in a way that the reader can easily understand the information presented without referring to the text of your report.

Discussion [Page limit – 1 – 1 ½ pages]

The discussion section is where you report on the interpretation and conclusion of your results. This is your opportunity to demonstrate your ability to analyze, evaluate, interpret and reason effectively. The discussion should relate your findings to your original question, hypothesis (or hypotheses if you had more than one), and predictions, which means that you evaluate your results in terms of your original question/hypothesis/predictions and point out the biological relevance of your findings. Avoid redundancy between the sections, especially the ā€˜Results’ and ā€˜Discussion’, of the lab report.

In addition, you should generalize the importance of your findings, discuss ambiguous data, and relate your results to other published studies (i.e., results published in primary scientific literature). Is your work in agreement or in contrast with previously published work? You should also discuss any sources of experimental error or limitations. You should end your discussion by summarizing the main points that you want the reader to remember; you should provide closure for the report and by extension, the reader. You should also recommend specific areas of further research based on your results and the findings of other published studies.

It is imperative that you include properly formatted in-text citations to support all non-original ideas within your discussion. Failure to include in-text citations will result in a major grade penalty.

Acknowledgements [Page limit – 1 paragraph, optional]

The acknowledgements section is where you can choose to acknowledge people who contributed to your work in some way but do not fit the criteria to be included as authors. This is also where you would include information about funding sources.

References [Page limit – 1/2 – 1 page]

You must include at least three primary scientific literature sources (which you are responsible for finding) as well as the BIOA01 lab manual in the proper format (Name-Year System, CSE Style- see Section C of the FLR Information page). Further resources can be included in addition to the three required primary sources. This style combines in-text parenthetical citations with a reference list at the end of your report (Walker and Rapley 2009). The references should be organized in alphabetical order by the primary author’s surname (last name) – DO NOT alphabetize the names within each citation. Be consistent when writing journal titles – write all journal titles out in full (e.g., European Food Research and Technology) or all abbreviated (e.g., Eur Food Res Technol).

Tip: Complete the online Library Research module and associated quiz to help you find relevant primary resources.

See examples below and more by using library resource document included with other FLR files on Quercus. Remember to remove subheadings when preparing your reference list. Reference list should be a single alphabetized list.

Scholarly Journal Article (primary source)

Ma Q, Scanlan C, Bell R, Brennan R. 2013. The dynamics of potassium uptake and use, leaf gas exchange and root growth throughout plant phenological development and its effects on see yield in wheat (Triticum aestivum) on a low-K sandy soil. Plant Soil 373:373-384.

Scholarly Journal Article (primary source found on the internet)

Mattupalli C, Genger RK, Charkowski AO. 2013. Evaluating incidence of Helminthosporium solani and Colletotrichum coccodes on asymptomatic organic potatoes and screening potato lines for resistance to silver scurf. Am J Potato Res [Internet]. [Cited 20 June 2013.] Available from http://link.springer.com/content/pdf/10.1007%2Fs12230-013-9314-3.pdf

Scholarly Journal Article (review, not a primary source)

Miao Y, Stewart BA, Zhang F. 2011. Long-term experiments for sustainable nutrient management in China. A review. Agronomy for Sustainable Development 31:397-414.

Chapter in Book (not a primary source)

Denison RF. 2012. Selfish genes, sophisticated plants, and haphazard ecosystems. In Darwinian Agriculture: How Understanding Evolution can Improve Agriculture. Princeton (NJ): Princeton University Press. Pages 76-94.

Chapter in Book Series (not a primary source)

Fageria NK, Moreira A. 2011. The role of mineral nutrition on root growth of crop plants. Advances in Agronomy (Book series) 110:251-331.

Internet Resource (secondary or tertiary source)

Williamson RC. 2004. Deciduous tree galls [Internet]. Madison (WI): University of Wisconsin-Madison; [cited 2013 Sep 12]. Available from http://labs.russell.wisc.edu/pddc/files/Fact_Sheets/FC_PDF/Deciduous_Tree_Galls.pdf

 
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External Forces And Their Impact On Health Care

External Forces and Their Impact On Health Care

Ā 

Health care quality and safety are not solely dependent on the actions of individual providers and institutions. A host of external forces exert a profound influence on what happens within any single organization or the behavior of any individual provider. These external forces include accreditation bodies, regulators, legislatures, insurers, and many other entities. Sociopolitical forces, including the economy and public opinion, also play a role in how and how well health care is provided.

 

To prepare for this Discussion Question:

 

Ā· Review this week’s Learning Resources.

 

Ā· Choose a specific example of an external force that influences health care and safety, as discussed in Chapter 2 of your Course Text.

 

Then, analyze how it influences health care quality management. (The external force can have minimal or extensive impact on quality.) Finally, evaluate whether the impact on health care is positive or negative, providing evidence to support your position.

 

 

PAPER

 

Pay-for-Performance

 

The predominant model for the delivery of health care in the United States and other parts of the world is fee-for-service. A new model gaining in popularity is known as pay-for-performance, or P4P. In the P4P model, providers are paid for how well they provide care, not how much care they provide. There are rewards for high quality, efficient and effective care and penalties for wastefulness and medical errors. Whether or not P4P can raise the standards of care and/or lower its cost is a matter of some disagreement.

 

To prepare for this Application Assignment:

 

Review the Learning Resources for this week that discuss pay-for for-performance.

Find two additional reputable sources (i.e., news sources, accreditation and health care agencies, peer-reviewed journal articles, etc.) that address the challenges of adopting a pay-for-performance approach for ensuring quality and safety in health care.

 

To complete this Application Assignment, write a 3-page paper that addresses the following:

Summarize and analyze the challenges discussed in the two sources you selected.

Select the two most significant challenges to the successful adoption of a P4P approach, and explain why.

 
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Outcome Of Both Vertical And Lateral Gene Transfer

1. Which describes the outcome of both vertical and lateral gene transfer? leads to rapid evolution of a population increases variability in a population results in large changes to the genetic code occurs between nonrelated organisms are types of mutation 2. Which of the following occurs in vertical gene transfer, but does not occur in horizontal gene transfer? a type of asexual reproduction rapid evolutionary changes the use of a sex pilus interaction between parent and offspring plasmid transfers 3. Which of the following best describes the role of vertical gene transfer in evolution? The types of genes passed on in vertical gene transfer are determined by artificial selection. Vertical gene transfer is not affected by fitness. Vertical gene transfer involves sexual reproduction that increases variation. Mechanisms of evolution do not act on processes of vertical gene transfer. Organisms that utilize vertical gene transfer have an increased chance of mutation. 4. Which of the following is not part of the process of bacterial conjugation? plasmids F+ cells F– cells sex pilus bacteriophage 5. Based on the role of gene transfer in populations, which process will not increase variability in a population? sexual reproduction conversion conjugation transformation transduction
 
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