SC160 Basic Biology Assignment 3 Essential Biology Discussion

Directions: Be sure to save an electronic copy of your answer before submitting it to Ashworth College for grading. Unless otherwise stated, answer in complete sentences, and be sure to use correct English, spelling and grammar. Sources must be cited in APA format. Your response should be four (4) double-spaced pages; refer to the “Assignment Format” page located on the Course Home page for specific format requirements.

 

 

Complete Question Text: Integrate by discussion the properties of life, basic chemical terminology, and molecules and compounds of a cell necessary for life. Include the basic anatomy and physiology of a cell and describe how cell respiration, photosynthesis, and cell reproduction occur in a succinct manner. Include a brief discussion about Mendel’s Laws and an overview of DNA structure and function. Conclude with a discussion of cancer and the mechanisms of gene control

 
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Drosophila Lab Report

Drosophila lab report.

1) I will upload for you the drosophila lab report Rubric.

2) I will upload the data of F2 phenotype.

3) the lab report expected ratios:

independent assortment 9:3:3:3

Genetics linkage 9:3:3:1

x linked / autosomal 2:1:1 ( female wt : male wt: male mutant )

3:1 ( wt : mutant ) apterous.

4) I will upload an introduction for this lab report but at the same time there is a lot of mistakes , you have to read the comment and correct them.

be careful please about the plagiarism and the read the citation required

not any citation be allowed, there is a required citation like peer review , journal …………………

1

 

Sandy Yossef Comment by Elizabeth Jackson: Grade:-2 hypotheses-2 chromosome locations-2 mutant lines-1 citation formatting-1 missing lab manual and flybase sources-1 grammar/italics6/15

Genetics lab

BIOL 3251-010

Assignment 1

Drosophila melanogaster, the common fruit fly, is a well-known model organism used for genetic research. The laboratory exercise will aid in differentiating normal ‘wild type’ and various mutant phenotypes of the fruit fly. The laboratory exercise will help in developing an understanding of the connection between the presence and absence of genetic trait of the general fruit fly population. Comment by Elizabeth Jackson: Drosophila melanogasterItalicize scientific names.Also, you should start with a statement about genetic inheritance. Comment by Elizabeth Jackson: It is too early in the introduction to talk about your own project. Focus on background information first.

Genetic science has identified and documented over 2,500 species of Drosophila. Out of these numbers of Drosophila one specie; Drosophila melanogaster (Fruit fly) has been primarily exploited as a model for genetic research. The D. melanogaster is the most suitable model for genetic work because of its short life cycle. This species lives for about 10 to 11 days and its optimum survival temperature is 220 C (Wangler, Yamamoto & Bellen, 2015). Another primary model advantage is that D. melanogaster has high reproductive potential. The species reproduces a large number of progeny. Such a large number of progeny is required for statistical analysis of results (Wolf, & Rockman, 2008). The general characteristics of genetic model organisms include a short generation time, a large number of progeny and easy genetic manipulation. Comment by Elizabeth Jackson: You need a citation for this.

Genetic scientists still have numerous biological processes to discover. The D. melanogaster has many possibilities because this model can quickly and efficiently answer questions of biological phenomena (Wangler, Yamamoto & Bellen, 2015). The D. melanogaster is an excellent model because it has a translational impact for genetic disease and a large number of medical implications including vector-borne illnesses. Comment by Elizabeth Jackson: You need citations for sentences like this.

This laboratory will use a wild-type D. melanogaster. Both male and females will use. The two sexes are differentiated in ways of physical characteristics. For example, males have a sex comb, a fringe of black bristles on their forelegs (Wolf, & Rockman, 2008). The male’s abdomens are elongate and rounded in males, but characteristically pointed in females. The wild type is the naturally occurring fly with not mutation. Other mutant flies that are common in the laboratory during breeding and that needs to be isolated from the wild-type ebony mutants that exhibit a much darker body as compared to the wild type fly (Wolf, & Rockman, 2008). Other common mutants are referred to as white-eyed female, wild type heterozygote female, white-eyed male, and wild type male. Comment by Elizabeth Jackson: Did we use this as a method for sexing the flies? Comment by Elizabeth Jackson: We didn’t look at ebony mutants in this lab. Comment by Elizabeth Jackson: This sentence doesn’t really make sense. You say “other common mutants”, but then you mention wildtypes. By definition, wildtypes are the exact opposite of mutants.

Flies with shortened wings are referred to as having vestigial genes that occur in the second chromosome. The vestigial genes occur due to recessive vg allele that specifies short vestigial wings. The flies have a recessive mutation of vestigial genes that occur in the second chromosome for both parents (Wolf, & Rockman, 2008). Other flies exhibit curled wings resulting from curly genes that also occur in the second chromosome. The flies those are yellow than normal exhibit yellow genes resulting from mutations occurring in the X chromosome (Wolf, & Rockman, 2008). The dark bodied flies carry a defect in their eye resulting to flies with phenotypic ebony genes. The ebony genes occur in the third chromosome and are responsible for building up a tan-coloured pigment. Comment by Elizabeth Jackson: Did you use flybase for this? If so, you need a citation. Comment by Elizabeth Jackson: We didn’t look at curled wings.

Complete and incomplete linkages in D. melanogaster occur independently. For example, the grey body and the long wing phenotype of this fly dominate over the black body and vestigial wings characteristics. Crossing over of genes occurs in the first generation, but the phonotype does not continue to the second generation (Garud, Messer, Buzbas, & Petrov, 2015). However, if the cross-over continued to the next generation it could be referred to as a complete linkage. Complete linkage in D. melanogaster genes is a rarity among female phenotype, but is common among the male mutant phenotype. The male mutant genes are closely associated and always transmitted together.

Reference

Garud, N. R., Messer, P. W., Buzbas, E. O., & Petrov, D. A. (2015). Recent selective sweeps in North American Drosophila melanogaster show signatures of soft sweeps. PLoS genetics11(2), e1005004. Comment by Elizabeth Jackson: The first authors of your citations are correct, with their last names first, followed by their first and middle initials. The other authors should have their initials first, followed by their last names. Comment by Elizabeth Jackson: Drosophila melanogaster

Wolf, M. J., & Rockman, H. A. (2008). Drosophila melanogaster as a model system for the genetics of postnatal cardiac function. Drug Discovery Today: Disease Models5(3), 117-123.

Wangler, M. F., Yamamoto, S., & Bellen, H. J. (2015). Fruit flies in biomedical research. Genetics, genetics-114.

 
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Microbiology Lab

2420-Lab 3- Bacterial Staining Techniques-II-(Differential)

Directions:

Answer following questions after reading the information and watching the video from the link below. Use color RED or BLUE for your answers. Submit the completed document on eCampus for grading. Refer to

· the textbook chapter 3 (3.2) (Nester- McGraw Hill)

· LinkBacterial Staining Techniques-IIVirtual Edge Experiment-4

Differential Staining

1. What is differential staining?

Gram Staining (4A on Virtual Edge)

Watch the video of Gram Staining from the textbook chapter 3- review 3.2 (pages 53-54), 3.5 (pages 65-67– cell wall of prokaryotic cells) and answer the following questions.

2. What is the purpose of Gram staining?

3. Who invented the Gram staining procedure?

4. What is the first stain used in Gram staining procedure?

5. Do all cells pick up this stain?

6. What is the function of a mordant?

7. Which mordant is used in the Gram staining procedure?

8. What would be the result if the mordant is forgotten from the procedure?

9. What is alcohol used for?

10. Why is decolorization necessary?

11. What is a counterstain?

12. Which counterstain is used in Gram staining?

13. What are cells called that retain the primary stain?

14. What are cells called that lose the primary stain?

15. What are cells called that take up the counterstain?

16. What type of cells do not take up the counterstain?

17. After the Gram staining procedure, Gram positive bacterial cells appear ______________ colored.

18. After the Gram staining procedure, Gram negative bacterial cells appear ______________ colored.

19. Which component of the cell wall in Gram positive bacteria is responsible for the retention of the primary stain?

20. Which component of the cell wall in Gram negative bacteria is responsible for the retention of the counterstain?

21. What would be the color of Gram negative bacterial cells if the decolorization step is forgotten? Explain.

22. Which bacteria were used in this experiment and what were their shapes? (From the textbook, review 3.3- Morphology of prokaryotic cells)

23. From what you have learned from the textbook, if a patient has E. coli infection which are Gram negative bacteria, would you advise them to take a course of Penicillin? Why or Why not?- Explain

24. Following picture shows the Gram staining result. Point out (or describe) Gram positive and Gram negative cells from the picture. Be sure to specify morphology:

Gram stain - Wikipedia

Acid-fast Staining- (4B on Virtual Edge):

Read the text from the following to help answering the following questions.

· link Acid-fast Staining,

· textbook chapter 3- section 3.2

25. List two genera of bacteria that are stained for identification by using Acid-fast staining method.

26. Which fatty acid is found in the cell walls of acid-fast bacteria?

27. Which primary stain is used in the acid-fast staining procedure?

28. Why is steaming necessary at this step?

29. Which counterstain is used in the acid-fast staining procedure?

30. Why is acid-alcohol used to decolorize in the acid-fast staining process instead of regular ethanol?

31. Which two types of bacteria were used in this experiment?

32. Which of the above two types of bacteria were acid-fast? (or retained the primary stain?)

33. From the picture below, point out the acid-fast cells:

Acid Fast Staining Flashcards | Quizlet

34. If you are working in a clinical diagnostic laboratory, which of the above staining methods would you use to predict the cause of infection under following conditions?

a. Tuberculosis

b. Strep throat

 
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Natural Selection and Guppy Evolution

Assignment: Natural Selection and Guppy Evolution

According to the theory of natural selection, organisms with traits that give them a greater chance of survival are more likely to pass these traits to offspring than organisms whose traits are not especially suited for survival. When Charles Darwin sailed on his 5-year-long voyage onboard the HMS Beagle, he recorded many observations of nature. Among these records, Darwin noted variations in beak shape and size among the finch populations throughout the Galápagos Islands.

Scientists who studied these beak variations realized the differences were not random, but related to the environment in which the finches lived. They discovered that finches tended to have beak types that made it easier for the birds to eat the foods growing in their particular environment (Belk & Maier, 2013). In what type of environment might finches with large, strong beaks have a greater chance of survival than finches with small, pointed beaks? Why might the finches with the smaller, pointed beaks be more likely to survive in a different type of environment?

Like Darwin and other scientists, biologist John Endler was interested in learning how environmental changes might influence the expression of advantageous traits in particular populations. Endler applied the principles of natural selection when he developed an experiment to study changes in guppy populations. He placed guppies in different environments and predicted how the fish populations would change in order to survive in those environments. For this week’s Assignment, you perform a virtual experiment based on John Endler’s experiment. Like Endler, you hypothesize how guppy populations will change to improve their chances of survival. You complete and submit a lab report for this Assignment.

To prepare for this Assignment:

  • Review Chapter 12 “An Evolving Enemy” and focus on the following:
    • What the theory of natural selection asserts
    • What evidence supports the theory of natural selection
    • How the theory of natural selection relates to the theory of evolution
  • Log on to MasteringBiology (see the Media section of the Required Resources) to review the animation “Principles of Evolution” and focus on the relationship between natural selection and evolution.

The Assignment:

By Day 5
  1. Print and save to your computer the Natural Selection Lab Report document from the Natural Selection Virtual Lab. You may complete this report by hand as you complete the lab; however, by Day 7, you submit an electronic version of this document for your Assignment.
  2. Complete the Natural Selection Virtual Lab. See the Required Resources section for the link to this activity.
    • Fill out appropriate sections of the Natural Selection Lab Report as you complete the lab. You may complete the analysis and conclusion sections of the lab report after you exit the virtual lab.

Questions about this Assignment? Post the questions in the Contact the Instructor area, so all class members may benefit from the Instructor’s response.

Refer to the Week 5 Assignment Rubric for grading details.

By Day 7

Following the instructions below, submit your Assignment. In order to receive full credit, all Assignments are due on time. Should you encounter an unanticipated and uncontrollable life event that may prevent you from meeting an Assignment deadline, contact the Instructor immediately to request an extension. Contact information for your Instructor can be found under the Contact the Instructor area. For a full description of the late policy, please refer to the “Policies on Late Assignments” section of your Syllabus.

Submission and Grading Information

To submit your completed Assignment for review and grading, do the following:

  • Please save your Assignment using the naming convention “WK5Assgn+last name+first initial.(extension)” as the name.
  • Click the Week 5 Assignment Rubric to review the Grading Criteria for the Assignment.
  • Click the Week 5 Assignment link. You will also be able to “View Rubric” for grading criteria from this area.
  • Next, from the Attach File area, click on the Browse My Computer button. Find the document you saved as “WK5Assgn+last name+first initial.(extension)” and click Open.
  • If applicable: From the Plagiarism Tools area, click the checkbox for I agree to submit my paper(s) to the Global Reference Database.
  • Click on the Submit button to complete your submission.

http://mym.cdn.laureate-media.com/2dett4d/Walden/BIOL/1001/CH/mm/virtual_lab_3/index.html

Natural Selection Lab Report

Name: ________________________

Date: _________________________

Updated 05/15/2014

Purpose: To apply the principles of natural selection to the effect of predation and background on the color patterns of male guppies over a period of 15 generations.

Natural Selection Virtual Lab Schedule

Note: You will complete the online Natural Selection Virtual Lab and this lab report in the following order:

1. Link to the online Natural Selection Virtual Lab and follow the lab instructions. Stop when the STOP sign appears in the virtual lab.

2. Complete Step 1 in the Results section of this lab report.

3. Complete the Hypothesis section of this lab report.

4. Return to the virtual lab and finish conducting the virtual experiment.

5. Record your observations from the virtual experiment by completing Step 2 in the Results section.

6. Complete the Analysis and Conclusion sections of this lab report.

7. Submit the finished lab report to your Instructor, following the submission instructions in your online classroom.

Hypothesis (5 points)

Note: Complete Step 1 in the Results section first. The observations you make there are ultimately part of the results and contribute to your hypothesis. Then, write your hypothesis (see instructions below).

In the table below, explain what you expect to observe in the color patterns of the male guppies in each of the ponds based on what you know about natural selection.

Pond 1 Prediction  
Pond 2 Prediction  
Pond 3 Prediction  
Pond 4 Prediction  

Note: After completing Step 1 in the Results section and writing your hypothesis, above, return to the Natural Selection Virtual Lab and select the NEXT button to complete the virtual experiment.

Materials and Methods

Before running this experiment you will create four different experimental ponds. The ponds will vary according to the kind of gravel (large, coarse gravel or small, fine gravel) and according to whether a guppy predator is present. In order to set up the experimental ponds you need to “drag and “drop” the correct sand size and the predators into the appropriate pond. You will then view a representative of a typical male guppy from a common pool and place a breeding population of these guppies in each of the experimental ponds. After approximately 15 guppy generations pass, the simulator will allow you to view a representative male guppy from each pond. You will observe and then record the male guppy color pattern representative of each pond in the results table and note any variations from the common ancestor.

Results (16 points)

Step 1:

a. Record your observations of the guppy color pattern before the start of the experiment in the table above. Describe a typical male guppy at the beginning of the experiment here:

b. Complete the Gravel Type and Predator columns in the table below.

Note: After completing Step 1, complete the Hypothesis section.

Step 2: After you select the TIME button in the interactive virtual experiment, record your observations of the changes in the color patterns of the guppies in the Observations Over Time column.

  Gravel Type

(coarse / fine)

Predator

(yes / no)

Observations Over Time.

Note the color pattern of typical male guppies in each pool and how they differ from that of their common ancestor.

Pond 1      
Pond 2      
Pond 3      
Pond 4      

Analysis (25 points)

Write a 3+ paragraph analysis of your results that includes the following:

· A summary of the observations of the changes in the guppy populations in each pond.

· A statement that explains whether you observed the results you expected, as well as why you did or did not observe the expected results.

· Explicitly describe the role of natural selection and the selection pressures present in this experiment.

· A statement explaining how, if at all, you think coloration in the male guppy populations in Ponds 1 and 2 would change over time if the predators were permanently removed (Note: Explain your hypothesis in terms of selection pressures.)

· A summary of the overall knowledge you gained from this lab

Conclusion (4 points)

Write a conclusion of one or more paragraphs that includes the following:

· A brief recap of the main points in your analysis

· Propose a question about this lab topic that you would like to research in the future

Natural Selection Lab Report – 1

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