Unknown Project (Microbiology)

Unknown Project Results Sheet Unknown #____
Gram Reaction ________________

Acid-fast Reaction ________________

Endospore ________________

Cell morphology ________________

and arrangement

Description of the colony:

Color _________________
Unique Characteristics ___________
Growth Characteristics:

Oxygen requirements? _____________
Biochemical tests: (+ or -)

Catalase _______ Phenylalanine deaminase ________
Oxidase _______ Decarboxylase tests:
Indole _______ Arginine ________
Methyl Red _______ Lysine ________
Voges-Proskauer _______ Ornithine ________
Citrate _______ Casein hydrolysis ________
Motility _______ Starch hydrolysis ________
Hydrogen sulfide _______ Lipid hydrolysis ________
Phenol red sugars: DNase ________
Glucose (gas) _______

Glucose (no gas) _______

Lactose _______

Sucrose _______

Mannitol _______

Gelatin hydrolysis _______

Urea hydrolysis _______

Nitrate reduction _______

The bacterium was identified as ____________________

(Genus and species)

Add description of your unknown here:

Name of test

Purpose/Biochemical Reaction

Name of Medium

Indicator or Reagent

Positive Reaction (visible reaction)

Negative Reaction (visible reaction)

Casein hydrolysis

Catalase test

N/A

Citrate test

Blue slant

Green slant

Deaminase test

Decarboxylase test

DNAse

Gelatin hydrolysis

H2S production

Indole test

Lipid hydrolysis

Methylene blue (indicator)

Methyl red test

Motility (from TTC)

Nitrate reduction

Oxidase test

N/A

Phenol red sugar broths (various sugars)

Starch hydrolysis

Urea hydrolysis

 
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Experiment The Properties of Water

Experiment The Properties of Water

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The Properties of Water

Margaret E. Vorndam, M.S. Version 42-0129-00-01

 

Lab Report Assistant

This document is not meant to be a substitute for a formal laboratory report. The Lab Report Assistant is simply a summary of the experiment’s questions, diagrams if needed, and data tables that should be addressed in a formal lab report. The intent is to facilitate students’ writing of lab reports by providing this information in an editable file which can be sent to an instructor.

 

Exercise 1: Surface Tension Results

 

D. State your hypothesis. What do you think will happen?

 

Data Table 1: Results of Surface Tension Trials

Trial # Paper Clip Weight, grams Does it Float? Yes/No

1

2

3

4

5 Compacted

6 + Detergent (optional)

 

 

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E. Record the results of your trials in the Data Table 1 above.

F. Did the weight of the paper clip affect whether it floated or not?

G. Did the size of the paper clip affect whether it floated or not?

H. Did the shape of the paper clip affect whether it floated or not?

I. Optional: Did the addition of detergent influence the flotation of the paper clip? Explain.

 

Questions

A. Why must the paper clips, tweezers, and bowl be clean?

B. If one paper clip did not float, what might be a reason?

 

 

C. Was your original hypothesis supported or refuted?

 

Discussion

A. Give two examples where the surface tension of water is important. Why is it important in these examples?

 

 

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Data Table 2: Results of Capillary Action Exercise

Capillary Tube Internal Diameter, mm Height of Liquid, cm

 

 

 

 

 

B. Would other liquids have the same surface tension property? Why or why not? How might you test this (for instance, the behavior of a paper clip and olive oil)?

 

C. Is there a limit to the tensile strength of the water surface? Explain.

 

D. What experimental variables are important to consider when doing this exercise?

E. What is the purpose of this exercise?

 

Exercise 2: Capillary Action Results

 

A. State your hypothesis. What do you think will happen?

B. Record the results of your exercise in the Data Table 2.

 

 

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C. Record the average results of at least three other students’ exercises in a table like Table 3. Why is it important to note how many data sets (N) are used in the average?

 

N (number of data sets averaged) =

Data Table 3: Results of Capillary Action Exercise

Capillary Tube Internal Diameter, mm

 

Height of Liquid, cm

 

 

 

 

D. Graph your data results from Tables 1 and 2 in a scatter graph, line graph, or bar graph similar to the graph layout in Figure 1. Also present the average results of the other students’ data using a second scatter, line or bar on the same graph so that the results of both your data and other students’ data can be compared. The graph should present two series of data, your results and the average students’ results, with Capillary Tube Internal Diameter on the x-axis, and height of liquid on the y-axis. Note: Do the graph by hand, or you may use a graphing program, and copy the resulting graph to your work area. Place your graph here:

 

E. What is the advantage of comparing your data to that of the other students’ data? Which set of data would you trust the most? Why?

 

 

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My data from Data Table 1 is recorded in (student to specify color of line) Other students’ average data from Table 2 are recorded in (student to specify color of line)

 

Questions

A. What did you observe at the end time of the exercise?

B. What caused the difference in the results that you obtained?

 

C. How did your outcome compare to that of the other students?

 

 

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D. Was there a linear relationship between Internal Diameter and the height of the water? What might explain this?

 

E. Was your original hypothesis supported or refuted?

 

 

Discussion

A. Give two examples where the capillary action of water is important. Why is it important?

 

B. Would other liquids have the same property? Why or why not? How might you test this?

 

C. Blood in our bodies travels through capillaries of differing internal diameters. This means that our heart can work less hard, since blood contains water as part of the fluid, and the capillary action of water in tubes helps to move the blood along.

 

1. Why does blood pressure rise as patients experience the progression of atherosclerotic disease?

 

Does this seem to be counter to what you have learned about capillary action? How do you explain the apparent discrepancy (note: see also c., below)?

 

 

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2. In plants, vascular system elements called xylem and phloem are lined up like long straws from the root of the plant to the leaves. The xylem of plants moves water and dissolved nutrients up to the leaves. Can you suggest what aids the movement of water up the plant, in addition to the capillary action of water?

 

3. Why is it harder to suck a beverage through a small diameter straw than through a large diameter straw?

 

4. What experimental variables are important to consider when doing this exercise? What could explain the difference in outcomes between your data and the data of other students?

 

5. What is the purpose of this exercise?

Exercise 3: Density Results

 

A. State your hypothesis. What do you think will happen?

 

B. Record what actually happened here:

 

 

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Questions

A. What did you observe in this exercise?

B. How does this outcome compare to what you observe about the action of ice (the solid form of water) on liquid water?

 

C. Was your original hypothesis supported or refuted?

Discussion

A. Why is it important that ice floats?

B. Give two examples where the property of water density is important.

C. Do all solids float on their liquid forms? Why or why not?

 

D. The density of liquid olive oil is about 0.92. What can you say about the likely density of solid oil? Why?

 

E. Will olive oil float or sink if it is added to liquid water? Why?

F. What experimental variables are important to consider when doing this exercise?

 

G. Attempt to find another pure substance, like water or oil, in which the solid form floats on the liquid form (Note: do not spend more than 5 minutes on this question!).

 

H. What did you learn from this exercise?

 

 

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Exercise 4: Specific Heat Capacity Results

 

A. State your hypothesis. What do you think will happen?

B. What difference do you observe in the heights of the frozen solid oil and water as compared to the liquid forms prior to freezing? What might explain this result?

 

 

C. Record what actually happened in a table similar to Tables 4 and 5.

 

Data Table 4: Results of Specific Heat Exercise for Water

Ambient (Room) Temperature in Which Exercise was Conducted, oC.

Time, minutes Liquid Collected, mL

 

 

 

 

 

 

 

 

 

 

 

 

All solid ice gone

 

 

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Data Table 5: Results of Specific Heat Exercise for Oil

Ambient (Room) Temperature in Which Exercise was Conducted, oC.

Time, minutes Liquid Collected, mL

 

 

 

 

 

 

 

 

 

All solid oil gone

D. Graph your data results from Data Tables 4 and 5. Use a scatter graph or progressive line graph, with time noted on the x-axis, and mL of liquid collected on the y-axis. You should have two scatter plots or lines on your graph (not two separate graphs) —one for water and one for oil. Note: you may use a graphing program, and copy the resulting graph to your work area. Place your graph here:

 

 

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E. Calculate the melting rate for each of water and oil. The melting rate is equal to the slope of each of the separate lines of your graph, one for water, and one for oil. The easiest way to calculate the slope for each is to use your graphing program, and ask it to determine the trendline for you, as follows:

 

 

F. Share your melting rate for water and the oil with other students, if possible. Make sure to also obtain the ambient (room) temperature from the other students. Why?

 

 

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Data Table 6: Comparison of Melting Rates for Specific Heat Exercise

 

Results Reported By

Water, mL/min

Oil, mL/min General Ambient Temperature, oC.

Student

Student A

Student B

Student C

Student D

Average =

Questions

A. Was your original hypothesis supported or refuted?

B. What do you observe concerning the plot line of the oil versus the plot line of the water? What does this mean?

 

C. How close is your melting rate to the melting rates reported by at least three other students for the water and for the oil?

 

D. Generally, what might you conclude about the amount of specific heat needed to melt 50 mL of water versus 50 mL of oil?

 

E. How could you explain the differences in your data observations as to when the water and oil began to melt?

 

 

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F. What experimental variables are important to consider when doing this exercise? What could explain the difference in outcomes between your data and the data of other students?

 

 

Discussion

A. How might you design a similar experiment to determine whether the specific heat (amount of heat required to raise 1 gram of a material by 1 ÂşC) of one substance is more or less than another substance? What variables must you consider in the design of your experiment? Give an example of such an experiment that compares the specific heats for two different substances.

 

B. An alternative energy-savvy person decides to use 55-gallon drums filled with liquid to warm her house at night in the winter. She has to make a decision about what liquid will be the most efficient at storing heat from sunlight that shines on the drums during the day. Should she use water or oil in the drums? Why?

 

Laboratory Summary

What have you learned from doing this laboratory?

 

 
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Genetics Review

Genetics Review

 

This assignment will make up for the two genetic labs that we would of done if we were meeting face to face. I will have you do various things to help you grasp the material. We would of done A LOT of Punnett squares in lab. If you are having trouble please contact me.

 

1. Match the correct definition to the term in the table below. Type the letter in the table.

 

A. Alternative form of a gene, located at a specific point on chromosome. (DNA coding that will determine distinct traits)

B. Having two identical alleles for a given gene

C. A unit of hereditary information with a specific sequence in DNA.

D. Having two different alleles for a given gene.

E. Breeding an organism of an unknown genotype with an organism with a homozygous recessive genotype. The offspring phenotype will determine the unknown genotype.

F. The genetic makeup or set of alleles of an organism

G. Allele’s phenotypic effect is not observed in a heterozygote

H. Allele’s phenotypic effect is fully expressed in a heterozygote

I. A cross done to determine if a gene is located on an autosome or sex-chromosome.

J. The physical traits of an organism determined by genetic makeup.

 

Term Definition Letter
Gene  
Allele  
Genotype  
Phenotype  
Homozygous  
Heterozygous  
Recessive Allele  
Dominant Allele  
Test Cross  
Reciprocal cross  

 

 

 

 

Star Wars Genetics – Monohybrid Crosses

 

Watch the following video to review on Monohybrid crosses.

https://www.youtube.com/watch?v=i-0rSv6oxSY

 

Geneticists at Endor have been investigating the genetic makeup of the organisms in this community. You will work through a few problems to help them out.

 

2. Brown body color (B) is dominant to red (b). Chewbacca recently met Susiebacca at a dance. They don’t have to worry about social distancing there right now. Chewbacca is heterozygous for his brown hair, but Susiebacca has red hair. Fill in the Punnett square below to see the possibilities if the night went well and they were to have children.

 

On the table below type in your answers. The black square will be left open. The parental gametes will be in the gray squares. There should only be one letter in each of these squares. The white squares are the possible kids. There will be two letters in those squares. Use this info for each time you see this type of table. Be sure to answer all of the questions. Some have two parts.

 

     
     
     

 

 

a. What are the possible genotypes for their children? What is the ratio?

 

b. What are the possible phenotypes for their children? What is the ratio?

 

 

3. Everyone in Anakin Skywalker’s family has “The Force” (A) which is determined by the amount of metacholorian in the body. Having “The Force” is a dominant trait. His family brags that they are a “purebred” line. He recently married a nice girl, Amidala, who does not possess “The Force” (a). Create a punnett square to show the possibilities that would result if Anakin and his new bride had children.

 

     
     
     

 

 

a. What are the chances of a child with “The Force”?

 

b. What are the chances of a child without “The Force”?

 

c. Would Anakin’s children still be considered purebreds? Explain.

 

 

4. Wicket W. Warrick, general of the Ewoks and his wife recently had a Lil’ Ewok. However… this has not been a happy occasion for them. Mrs. Warrick has been upset since she first saw her new baby who has stripes. She claims the hospital goofed and mixed up her baby with someone else’s baby. Mr. Warrick is homozygous for his solid-colored fur, while his wife is heterozygous for her solid-colored fur. Solid (A) is dominant to stripes (a) in fur. Do the Punnett square to see if the hospital messed up.

 

     
     
     

 

 

a. List the possible genotypes of their kids. What is the ratio?

 

b. List the possible phenotypes of their kids. What is the ratio?

 

c. Did the hospital mess this one up or is it possible that it is their kid? Explain.

 

 

Dihybrid Problem (only one I promise)

 

Watch this video to remind yourself about dihybrid crosses.

 

https://www.youtube.com/watch?v=qIGXTJLrLf8

 

 

5. In horses, the coat color black is dominant (B) over chestnut (b). The trotting gait is dominant (A) over the pacing gait (a). If a homozygous black pacer is mated to a homozygous chestnut, heterozygous trotter.

 

In this table you will not have anything in the black square. The parental genotypes will be in the gray squares. You will have two letters in each gray square. The potential babies are going to be in the white squares. There will be four letters in those squares.

 

         
         
         
         
         

 

 

 

a. What are the parental genotypes?

a.

b.

 

b. What are the gamete combinations you got from the FOIL method for both parents?

a.

b.

 

c. What are the genotypes of the offspring and the ratio?

 

d. What are the phenotypes of the offspring and the ratio?

 

 

Sex-Linked Problems

 

Watch the following video to review yourself on sex-linked traits.

https://www.youtube.com/watch?v=h2xufrHWG3E

 

Be careful when answering these questions. They will be asking things specific to gender. Remember that Sex-linked problems tell us both the gender and the genotype/phenotype.

 

6. In humans, hemophilia is a sex-linked recessive trait on the X chromosome. If a female who is a carrier for hemophilia marries a male with normal blood clotting, answer the following questions.

 

     
     
     

 

 

a. What fraction of the female children will have hemophilia?

 

b. What fraction of the female children will be carriers?

 

c. What fraction of the male children will have normal blood clotting?

 

d. What fraction of the male children will have hemophilia?

 

 

7. Color-blindness is a sex-linked recessive trait on the X chromosome. Two normal vision parents have a color-blind son.

 

     
     
     

 

 

a. What are the genotypes of the parents?

 

b. What fraction of the male children will be color-blind?

 

c. What fraction of the female children be carriers?

 

d. What fraction of the female children will be color-blind?

 

 

Blood Typing Problems

 

Watch the video to review yourself on blood typing.

https://www.youtube.com/watch?v=9O5JQqlngFY

 

8. Suppose a father and mother claim they have been given the wrong baby at the hospital. They must have been at the same hospital the Ewoks were at. Both parents are blood type A. The baby they have been given is blood type O.

 

     
     
     

 

 

Is it possible it is their kid? Work out the Punnett square and explain your reasoning.

 

 

 
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Health Care Finance Models

The purpose of this assignment is to describe how health care is financed by examining both the market-based and government-based approaches.

Access the “Health Care Financing” document and complete the comparison chart. Write 3-4 sentences minimum for each approach. Submit the complete document in the assignment dropbox.

Prepare this assignment according to the guidelines found in the APA Style Guide, located in the Student Success Center. An abstract is not required.

This assignment uses a rubric. Please review the rubric prior to beginning the assignment to become familiar with the expectations for successful completion.

A COMMUNITY OF LEARNERS’ 6

 

 

 

Health Care Finance Models

There are two broad approaches to financing health care: a market-based approach and a government-financed approach. Answer the 12 questions, providing 3-4 sentences minimum for each approach.

 

Include any references used at the end of the chart.

 

  Market-based

(HMO, POS, PPO, etc.)

Government-financed

(Medicare, Medicaid, Tricare, etc.)

Who is provided access?    
How much coverage is provided?    
How are the services paid for?    
How does reimbursement apply?    
Are there limitations of care?    
What guides care decision for patients?    
What is the quality of services (use HEDIS website for answer)?    
Are there competitive options?    
How much are prevention and wellness emphasized and measured?    
How are health care costs managed and controlled?    
How are medical advances generated?    
How is health care reform established?    

 

 

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